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Ultrasensitive, low-input detection of avocado sunblotch viroid via RPA-CRISPR and nanopore-array single-bead fluorescence readout.
PubMed · 2026-05-20
Scientists developed an ultra-sensitive, portable test for Avocado Sunblotch Viroid that can detect the pathogen at fewer than 2 copies per microliter from a tiny drop of plant extract — making early orchard diagnosis practical without a laboratory.
1
Detected Avocado Sunblotch Viroid at concentrations as low as 1.68 copies per microliter, representing one of the most sensitive viroid detection thresholds reported.
2
Used only 40 nanoliters of sample per readout — more than 100-fold less plant material than conventional fluorescence-based assays require.
3
The complete test runs in 60 minutes at a constant temperature with no PCR thermocycler needed, enabling potential deployment directly in orchards or the field.
