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Tracking antibiotic resistance genes and microbiome shifts under reclaimed wastewater irrigation: Root-associated selective modulation.

PubMed · 2026-04-17

Irrigating crops with recycled wastewater spreads antibiotic-resistant bacteria and genes into soil, but plant roots appear to act as a natural filter, significantly reducing how much of that resistance makes it inside the plant.

1

Antibiotic resistance genes (sul1, ermB, intI1) rose significantly in soils irrigated with treated and raw wastewater, but these increases were not mirrored inside plant roots.

2

Root microbiomes were more resilient than soil microbiomes to wastewater irrigation, with most wastewater-associated bacterial groups absent or reduced in roots compared to surrounding soil.

3

One plant pathogen genus (Rhizorhapis) was promoted in roots under raw wastewater irrigation, indicating the root barrier is selective but not absolute.