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Gold nanoparticle-mediated elicitation enhances andrographolide biosynthesis via auxin signalling in optimized hairy root cultures of Andrographis paniculata.
PubMed · 2026-05-22
Researchers grew medicinal plant roots in a lab and used tiny gold particles to dramatically boost production of andrographolide — a compound used in traditional medicine to fight infections and inflammation — achieving nearly 9 times more of it than normal.
1
Treatment with gold nanoparticles at 40 mg/L for 48 hours increased andrographolide production 8.8-fold, reaching 372.17 mg per gram of dry weight.
2
Hairy root cultures achieved a transformation efficiency of 79.9% and peak biomass on day 40, providing a reproducible lab-based production platform.
3
Gold nanoparticle elicitation activated auxin-signaling genes ARF1 (9.2-fold) and ARF3 (6.09-fold), revealing the hormonal pathway driving the yield boost.
