molecular-diagnostics

1 article

Exponential signal amplification through coupled rolling circle transcription and CRISPR/Cas13a cleavage for ultrasensitive molecular diagnostics.

PubMed · 2026-05-14

Scientists developed a fast, ultra-sensitive test called SPRINT-C that can detect a single molecule of genetic material in just 10 minutes using a chain of three coupled enzymatic reactions, including CRISPR technology.

SPRINT-C detects single molecules of nucleic acid in under 10 minutes using a one-pot isothermal reaction requiring no thermal cycling equipment

T7 RNA polymerase transcription rate (5.97 cycles/s) vastly outpaces LwCas13a cleavage rate (<0.019 s⁻¹), creating an exponential signal amplification cascade

Three enzymatic steps — padlock ligation, rolling circle transcription, and CRISPR/Cas13a cleavage — are seamlessly coupled in a single reaction vessel

molecular-diagnostics

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