Scientists developed a fast, cheap genetic test to detect fungicide-resistant strains of gray mold (Botrytis cinerea) and confirmed that the resistant mutations carry no fitness penalty, meaning resistant strains spread just as aggressively as susceptible ones.
1
Among 82 resistant Botrytis cinerea isolates from strawberry and tomato, 70.2% carried the L412F mutation, 8.3% the L412V mutation, and 4.7% the G408V mutation in the Bcpos5 gene.
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CRISPR/Cas9-generated mutants carrying L412F and L412V showed no significant fitness costs — their mycelial growth, spore production, and plant infection ability matched the non-resistant reference strain.
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A new T-ARMS-PCR test can rapidly and cheaply identify the three most common resistance mutations from a single DNA sample, validated against Sanger sequencing across 170 field isolates.
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