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Better genetic markers sort out look-alike Vibrio bacteria that standard barcoding blurs

PubMed · 2026-09-21

The standard DNA barcode used to identify bacteria (16S rDNA) blurs the differences between closely related Vibrio species, while sets of single-copy genes tell them apart much more sharply. Two genes, mfd and uvrC, stand out as better markers for tracking these bacteria in environmental samples.

1

Single-copy Vibrio genes produced more highly resolved phylogenetic trees than 16S and 23S rDNA sequences.

2

Single-copy loci showed relatively high congruence with one another, while rDNA phylogenies diverged from them (measured with Robinson-Foulds distances).

3

The genes mfd and uvrC were highlighted as top candidates for Vibrio evolution studies and environmental sample analysis; incongruence may stem from horizontal gene transfer or limited rDNA sequence variability.

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microbial-ecology phylogenetics molecular-markers