Field-Compatible Detection of Spiroplasma citri Associated with Citrus Stubborn Disease Using CRISPR-Cas12a and Crude Sample Preparation.
Shih J, Yokomi RK, Hajeri S, Roy A, Wei W
Crispr
Researchers developed a portable CRISPR-based test that detects citrus stubborn disease in the field within minutes using simple sample preparation, potentially transforming disease management from laboratory-dependent to on-site diagnostics.
Key Findings
1
CRISPR-Cas12a assay achieves detection limit of 1 attomolar (1.8 genome copies) with plate reader, 10 aM with lateral flow format
2
100% diagnostic accuracy compared to gold-standard qPCR when validated with extracted DNA from symptomatic citrus samples
3
10-minute crude extraction protocol (NaOH-Tris) enables equipment-free sample preparation with 70% accuracy in field-compatible lateral flow format
description
Abstract Preview
Citrus stubborn disease (CSD), caused by Spiroplasma citri, presents a significant risk to citrus production, resulting in considerable yield losses when infections remain undetected. Accurate and ...
Citrus is a genus of flowering trees and shrubs in the family Rutaceae. Plants in the genus produce citrus fruits, such as citrons, mandarins, and pomelos. Many important citrus crops have been developed through extensive hybridization, including oranges, lemons, grapefruits, and limes, all of wh...
