Field-Compatible Detection of
Shih J, Yokomi RK, Hajeri S, Roy A, Wei W
Crispr
Researchers developed a portable CRISPR-based test that detects citrus stubborn disease in the field within minutes using simple sample preparation, potentially transforming disease management from laboratory-dependent to on-site diagnostics.
Key Findings
CRISPR-Cas12a assay achieves detection limit of 1 attomolar (1.8 genome copies) with plate reader, 10 aM with lateral flow format
100% diagnostic accuracy compared to gold-standard qPCR when validated with extracted DNA from symptomatic citrus samples
10-minute crude extraction protocol (NaOH-Tris) enables equipment-free sample preparation with 70% accuracy in field-compatible lateral flow format
Abstract Preview
Citrus stubborn disease (CSD), caused by Spiroplasma citri, presents a significant risk to citrus production, resulting in considerable yield losses when infections remain undetected. Accurate and ...
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