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Two competing genes decide how long your cucumber grows

Zhang T, Han N, Li P, Zhang J, Zhang Q

Crispr

The next time you pick between a stubby pickling cucumber and a long slicing one at the farmers market, you're seeing the output of a genetic tug-of-war scientists just mapped for the first time.

Cucumber shape, whether round or long, comes down to a battle between two genes inside the fruit. One gene called CsSUN pushes the fruit to grow longer, while a calcium-sensing partner gene called CsCaM11 pulls in the opposite direction and shortens it. When researchers used gene editing to switch off CsSUN, cucumbers shrank by nearly half; when they switched off CsCaM11 instead, cucumbers grew almost 18% longer.

Key Findings

1

CsSUN carries a 161 bp deletion causing a frameshift mutation in round-fruited cucumber lines; CRISPR/Cas9 knockout of CsSUN reduced fruit length by 38-46%

2

Calcium ion levels were 15.2% higher in round-fruit (sun161) lines, linking calcium signaling to fruit shape

3

CsCaM11 physically interacts with and is epistatic to CsSUN: overexpressing CsCaM11 shortened fruit 5-7.5%, while knocking it out lengthened fruit by 17.9%

chevron_right Technical Summary

Scientists identified two genes that work against each other to control how long cucumbers grow, and editing either one can make cucumbers up to 46% shorter or nearly 18% longer. The discovery explains why some cucumber varieties are round while others are long, and offers a genetic tool for breeding cucumbers with the exact shape shoppers want.

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Abstract Preview

Original paper

IQ domain protein CsSUN interacts with the Calmodulin protein CsCaM11 to regulate fruit length of cucumber.

Fruit length is an important fruit quality which greatly influences the preference of consumers, but the underlying genetic mechanism regulating the fruit length in cucumber remains not very clear....

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Abstract copyright held by the original publisher.

hub This connects to 9 other discoveries — Cucumber crispr, crop-improvement, plant-signaling 5 related articles

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