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Deleting one rice gene makes plants far more salt-tolerant

Lei H, Zhu Y, Du Q, Jiang L, Quan R

Crispr

Salty soil is quietly taking farmland out of rice production worldwide, and knowing exactly which gene to switch off gives breeders a fast, precise route to varieties that can grow where salt would normally kill them.

Rice plants have a gene called SZF12 that normally acts like a brake, dialing down the plant's stress response when salty water hits its roots. When researchers used CRISPR gene editing to knock out this gene, the rice plants handled salt far better: they kept less sodium in their tissues, survived longer, and even grew more grain. Plants engineered to make extra SZF12 did the opposite, struggling under salt stress and growing poorly, confirming that this one gene is a key switch controlling how well rice copes with salty soil.

Key Findings

1

CRISPR/Cas9 knockout mutants (szf12-c1, szf12-c2) showed higher survival rates, lower Na+ accumulation, and lower Na+/K+ ratios under salt stress compared to wild-type rice

2

SZF12-overexpressing lines showed the opposite pattern: reduced salt tolerance, stunted growth, and altered grain length

3

Loss of SZF12 activated ion transport genes and suppressed carbon metabolism genes, enabling more targeted transcriptional reprogramming under salt stress, and knockout plants showed increased grain yield under salt stress

chevron_right Technical Summary

Scientists found a rice gene called SZF12 that actually holds back the plant's ability to fight off salty soil. Turning this gene off with CRISPR made rice plants survive salt stress better and even produce more grain, pointing to a promising target for breeding tougher, salt-resistant rice.

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Abstract Preview

Original paper

The CCCH-type zinc finger protein SZF12 negatively regulates salt stress tolerance in rice by suppressing stress-responsive transcriptional reprogramming.

Soil salinity represents one of the most damaging abiotic stresses constraining rice (Oryza sativa L.) production globally. Although CCCH-type zinc finger proteins have emerged as important regulat...

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Abstract copyright held by the original publisher.

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