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An InDel in the FAD5 promoter confers cold tolerance by enhancing transcriptional activation of unsaturated fatty acid synthesis in grapevines.

Zhou H, Li Q, Wong DCJ, Meng L, Hou Y

Climate Adaptation

Cold snaps that wipe out a vineyard overnight could eventually be countered with grape varieties bred from this genetic insight — giving growers in frost-prone regions a fighting chance without heavy infrastructure like wind machines or heaters.

Plants keep their cell membranes flexible in cold weather using a special type of fat — similar to how olive oil stays liquid in your fridge while butter goes solid. Researchers found a tiny glitch in the DNA instruction manual for grapevines that turns up production of these cold-weather fats, letting some vines weather freezing temperatures much better. Finding this genetic switch is a big step toward breeding wine grapes that can handle unpredictable winters.

Key Findings

1

A small insertion/deletion (InDel) in the regulatory region of the FAD5 gene was pinpointed as a driver of cold tolerance, identified using Bulked Segregant Analysis of a grapevine breeding population.

2

The InDel boosts FAD5 gene activity, increasing the proportion of unsaturated fatty acids in cell membranes — the key molecular mechanism keeping membranes functional at low temperatures.

3

The study establishes FAD5 as a candidate gene for marker-assisted selection of cold-hardy grapevine varieties, with implications for woody perennial crops broadly.

chevron_right Technical Summary

Scientists identified a tiny DNA insertion/deletion near the FAD5 gene in grapevines that ramps up production of unsaturated fatty acids in cell membranes, directly improving cold tolerance. This genetic variant explains part of why some grapevine varieties survive harsh winters better than others.

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Abstract Preview

The ratio of unsaturated fatty acids (UFAs) in membranes is tightly associated with plant cold hardiness; however, the genetic basis underpinning UFA biosynthesis in woody perennials remains poorly...

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hub This connects to 11 other discoveries — Grapevine climate-adaptation, crop-improvement, cold-tolerance +2 more 5 related articles

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Vitis (grapevine) is a genus of about 80 species of twining plants in the family Vitaceae. The genus consists of species predominantly from the Northern Hemisphere. It is economically important as the source of grapes, both for direct consumption of the fruit and for fermentation to produce wine....