Researchers edited several yield-related genes at once in the rice variety Kasalath and found that the results depend on which genes and which kinds of changes are combined, not on simple addition. One triple-edited line kept normal yield, while another gained bigger grains but lost plant height and total yield.
1
Of 28 first-generation plants, edits were detected in GS3 (35.71%), GW2 (10.71%), ROC5 (7.14%) and Gn1a (3.57%); TAD1 and LARGE2 showed no edits at this stage.
2
The gs3-13d frameshift allele increased tillering and panicle number but reduced grain length and yield, showing strong pleiotropic effects; the recovered GW2 alleles were intronic and left the coding sequence unchanged.
3
The roc5-7d gw2-8d gs3-3i triple mutant kept wild-type yield with altered leaf architecture, while gw2-8d gs3-3i large2-10d had higher grain width, thickness and 1000-grain weight but severe drops in height, tillering, seed setting and yield per plant.
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