OpenAlex:
Artificial Intelligence in Plant Sciences
OpenAlex:
Artificial Intelligence in Plant Sciences
OpenAlex:
sRNA_seq_clean_thrips_leafdiscs_timeseries
OpenAlex:
sRNA_seq_clean_thrips_leafdiscs_timeseries
OpenAlex:
Ugandan healers near Rwenzori Mountains use 95 plants as medicine
OpenAlex:
Color phenotyping and genome-wide association studies of ...
OpenAlex:
FUNCTIONAL STUDIES ON SIEVE ELEMENT-SPECIFIC PROTEINS
OpenAlex:
Understanding the control of perpetual flowering and cont...
OpenAlex:
Artificial Intelligence in Plant Sciences
OpenAlex:
Artificial Intelligence in Plant Sciences
OpenAlex:
sRNA_seq_clean_thrips_leafdiscs_timeseries
OpenAlex:
sRNA_seq_clean_thrips_leafdiscs_timeseries
Two-guide CRISPR tool makes gene knockouts in poplar trees far simpler
PubMed · 2026-07-05
Scientists developed a faster, more reliable method to knock out genes in poplar trees using a two-guide CRISPR system that deletes large chunks of DNA instead of making tiny edits, making it much easier to confirm the mutation worked.
1
Dual-gRNA CRISPR/Cas9 strategy reliably deletes genomic fragments larger than 50 bp, compared to the small indels typical of single-gRNA approaches.
2
Mutant trees can be identified by conventional PCR and agarose gel electrophoresis, eliminating the need for routine DNA sequencing during screening.
3
The protocol was validated using the PtFBX230 gene in Populus tremula x P. alba clone INRA 717-1B4 and is designed to be transferable to other loci and related woody species.
