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Virus detection in plant science refers to the methods and technologies used to identify viral pathogens infecting plant tissues, ranging from serological assays and PCR-based techniques to advanced next-generation sequencing approaches. Accurate and early detection of plant viruses is critical for protecting crops and ecosystems, as viral infections can cause significant yield losses, quality degradation, and the spread of disease to healthy plants. These diagnostic tools enable researchers and growers to implement timely management strategies, develop resistant cultivars, and monitor the emergence of new or evolving viral threats.

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Field-parallel six-sample microfluidic detection of plant viruses via raffinose-assisted one-pot LAMP-CRISPR/Cas12b.

PubMed · 2026-05-01

Scientists developed a portable device that can simultaneously test six plant samples for viral diseases in the field, using CRISPR gene-editing technology combined with a rapid amplification method. It matches the accuracy of laboratory-grade testing without needing a lab.

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The ALERT platform detected plant viruses with sensitivity comparable to conventional RT-PCR, the current gold-standard lab method.

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Field testing showed 100% diagnostic agreement with RT-PCR results across all samples tested.

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The portable device can process six samples simultaneously in the field using an integrated microfluidic chip and a compact metal incubator.