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A synthetic expression system for orthogonal gene expression in Nicotiana benthamiana.
PubMed · 2026-06-02
Researchers built a synthetic genetic 'volume knob' for plants that controls how much of any target protein a plant produces — and it outperforms the industry-standard tool, the CaMV 35S promoter, in both strength and consistency.
1
The synthetic expression system (SES) produced higher and more stable RNA transcript levels than the widely used CaMV 35S promoter in all tested conditions
2
SES functioned without needing p19, a helper protein typically required to prevent plant immune silencing of foreign genes in transient expression experiments
3
SES successfully drove high-level accumulation of four distinct proteins — mCherry, glucose oxidase, protein A, and human VEGF165 — and remained functional in both plant and fungal hosts
