Researchers built a tool from a mild pepper virus that switches off chosen genes in pepper plants and fruits within weeks, skipping slow genetic engineering. It worked in seven pepper varieties and helped boost resistance to two viral diseases.
1
The new PMMoV-based vector pKL736 produced strong GFP fluorescence in systemically infected leaves across multiple pepper cultivars.
2
A ~150 bp insert (pKL736-PDS156) gave optimal silencing, causing leaf photobleaching at 20 days post-inoculation and fruit silencing efficiencies of 90.7% (Zunla-1) and 85.5% (JS).
3
Silencing efficiency exceeded 80% in leaves and fruits of five additional varieties, and silencing eIF4E significantly increased resistance to two potyviruses.
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