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Identification of a novel BBWV2 isolate and a sensitive and rapid RT-RPA-CRISPR/Cas12a-LFD detection method development.

PubMed · 2026-02-11

Researchers identified a new strain of a virus that devastates vegetable crops and built a fast, portable test that can detect it in the field within an hour — no lab equipment needed.

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A new isolate of Broad Bean Wilt Virus 2 (BBWV2-GZCa) was discovered infecting peppers in Guizhou Province, China.

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The new RT-RPA-CRISPR/Cas12a lateral flow test detects as few as 7.5 virus copies per microliter — 100,000 times more sensitive than conventional RT-PCR.

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The assay achieved 100% accuracy on 20 field-collected samples and can be completed in under one hour with no specialized equipment.

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