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Development of an Efficient Regeneration and Agrobacterium-Mediated Transformation Protocol for Hosta 'Light Star' Using the RUBY Reporter Gene.

PubMed · 2026-04-20

Scientists developed the first reliable method to grow and genetically modify Hosta 'Light Star' in the lab, using a color-changing reporter gene that turns transformed plants red — making it easy to spot successful genetic edits without destructive testing.

1

Optimal callus induction medium achieved a 53.33% induction rate from leaf explants using MS medium with 6-BA, NAA, and 2,4-D.

2

Adventitious bud proliferation reached a coefficient of 5.87, and both rooting and transplant survival hit 100% under optimized conditions.

3

The RUBY reporter gene enabled non-invasive visual screening of successfully transformed plants by turning them red, simplifying the identification of positive transformants.