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BOTany Methods: Accessible Automation for Plant Synthetic Biology.

PubMed · 2026-02-19

Researchers created BOTany Methods, a free toolkit of automated lab protocols for small robots that lets plant scientists run complex genetic experiments without needing coding skills or expensive equipment.

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The BOTany Methods toolkit runs on Opentrons OT-2 robots, which are significantly cheaper than full biofoundry setups, lowering the barrier to lab automation for plant research.

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The workflow covers a wide range of tasks — from simple primer dilution and PCR setup to complex operations like Plant Modular Cloning and bacterial transformation — all without requiring users to write or edit Python code.

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The system supports users across all training levels, from undergraduate students to senior scientists, using table-based inputs to design and run custom experiments end-to-end.