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Robustness of microbial quantification methods to seawater in marine plastic biodegradation test.
PubMed · 2026-05-20
Researchers tested four methods for counting microbes in seawater and found that manual cell counting and quantitative PCR (a DNA-based technique) were the most reliable and consistent for studying how plastics break down in the ocean.
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Manual cell counting and quantitative PCR were both repeatable and reproducible in seawater environments.
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All four methods were evaluated for compatibility with seawater, but only two passed as reliable for biodegradation testing.
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The study recommends that marine plastic biodegradation tests should always pair results with a carefully vetted microbial quantification method.