nanopore-sequencing
Nanopore sequencing is a DNA and RNA sequencing technology that generates long, continuous reads of genetic material in real-time by analyzing molecules as they pass through protein nanopores. For plant research, this approach is particularly valuable because plants frequently have complex, polyploid genomes with large repetitive sequences that are difficult to sequence and assemble using shorter-read technologies. The long reads provided by nanopore sequencing enable researchers to better resolve genomic structure, identify structural variants, and construct more complete and accurate genome assemblies.
open_in_new WikipediaPubMed · 2026-02-17
This study develops techniques to identify which bacteria in microbiomes carry mobile genetic elements—genes that jump between microbes and spread traits like antibiotic resistance. The combined approach helps researchers track how antibiotic resistance spreads through bacterial populations.
Combined nanopore adaptive sampling, DNA methylation detection, and Hi-C sequencing can effectively identify bacterial hosts of mobile genetic elements in complex microbiomes
These methods require careful experimental design, interpretation, and validation despite their technical power
Validation using spiked samples with known bacteria demonstrated the approach's capability to track mobile element distribution