Scientists refined a freezing technique that lets researchers see the ultra-fine structure inside plant cells, including the lipid membranes of individual organelles, by figuring out how to properly remove trapped air from leaves and petals before freezing them solid.
1
1-hexadecene works as an effective cryoprotectant/filler for high-pressure freezing of plant tissues including Arabidopsis roots
2
Vacuum degassing of tissue in 1-hexadecene removes intercellular air bubbles, which is essential for successful freezing of vacuolated tissues like mature leaves and petals
3
Optimal degassing time correlates positively with tissue thickness, tested across Arabidopsis anthers, Brachypodium, Axonopus, Plantago leaves, and Phalaenopsis petals
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