microbial-enzymes

1 article

Purification and characterization of the laccase produced by the Lysinibacillus boronitolerans and its role in the decolourization of synthetic dyes.

PubMed · 2026-06-23

Researchers purified a dye-degrading enzyme from a soil bacterium and used it to break down toxic textile dyes, achieving up to 84.5% removal of Congo red under optimized conditions. This offers a greener alternative to chemical wastewater treatment.

The laccase enzyme was purified 12.19-fold with a 63.86% recovery rate and a molecular weight of ~45 kDa.

Congo red dye showed 61% degradation with crude enzyme, rising to 84.5% after optimizing conditions with Response Surface Methodology.

Enzyme activity peaked at pH 5 and 40°C, and was boosted by copper ions while being blocked by sodium azide.

microbial-enzymes

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