A step-by-step lab method lets researchers see exactly where specific proteins sit on maize chromosomes while sperm and egg precursor cells divide. Seeing this shows how corn shuffles its genes, which is the raw material for breeding new varieties.
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The protocol dissects anthers at specific meiotic stages, fixes them, and releases meiotic cells onto slides for antibody staining.
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Primary antibodies bind target proteins and fluorescent secondary antibodies reveal their location under a fluorescence microscope.
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The method can show protein colocalization and can be adapted to somatic (non-meiotic) cells.
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