Scientists knocked out a gene in tomato plants that normally suppresses pest defenses, producing plants that resist the devastating tomato leaf miner moth without any loss in growth or fruit yield.
1
Knocking out SlRaptor1A with CRISPR-Cas9 conferred enhanced resistance to Tuta absoluta (tomato leaf miner) with no reduction in plant growth or yield.
2
Mutant plants showed elevated levels of jasmonic acid, salicylic acid, and defensive secondary metabolites including alkaloids and phenylpropanoids during insect infestation.
3
Multi-omics analysis (transcriptomics + metabolomics) confirmed coordinated upregulation of defense genes and metabolite accumulation, identifying SlRaptor1A as a negative regulator of the plant's immune response.
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