Plants send internal signals, like waves of calcium, to react to touch, injury, and stress. This review explains which measurement and analysis methods reveal how those signals actually travel, and which ones miss important details.
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Live imaging now captures calcium signals in plants at the molecular level with high spatial and temporal resolution.
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Analysis methods for dynamic signals can reveal parameters set by the molecular machinery that generates and propagates the signal.
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No single analysis method reveals every type of information, so choosing the right method determines what mechanisms can be uncovered.
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