Scientists developed a method to genetically edit heirloom tomato varieties — the flavorful, non-commercial types prized by gardeners and farmers markets — using CRISPR, opening the door to improving these beloved varieties without losing what makes them special.
1
Six commercially relevant heirloom tomato cultivars were successfully tested for transformation and regeneration capacity, expanding beyond the four lab-standard varieties (M82, Ailsa Craig, Microtom, Sweet-100) that dominate existing protocols.
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Use of the GRF4-GIF1 chimeric developmental regulator significantly improved recovery of transgenic plants by enhancing regeneration efficiency.
3
CRISPR/Cas9 genome editing was validated in multiple heirloom cultivars, successfully producing edited plants with targeted changes to plant architecture and flowering time genes.
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