Scientists developed a highly sensitive dual-technology test (combining RPA and CRISPR) to reliably detect Cacao Mild Mosaic Virus, a pathogen that hides in cacao trees at low levels and often causes no visible symptoms, making it a hidden threat in breeding programs and germplasm collections.
1
CaMMV accumulates at low levels in cacao leaves and petioles, causing detection failures with conventional methods, particularly in certain cacao genetic groups
2
A multiplex RPA assay using three primers targeting two regions of the virus's movement protein was developed to increase detection reliability
3
Coupling RPA with CRISPR/Cas12a created a system capable of detecting the virus even during asymptomatic, seasonally variable infections
mail
Weekly plant science — one email, Saturdays.
Check your inbox to confirm — link expires in 24 hours.
Something went wrong — please try again.
Too many signup attempts from your network. Try again in an hour, or email hello@plant.news.
