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Glowing bacteria sensor speeds up building gene-silencing pest fighters
PubMed · 2026-07-06
Scientists built a glowing sensor that lets them fine-tune bacteria to churn out large amounts of double-stranded RNA, the molecule that can switch off specific genes in pests. This speeds up the design of microbes that could deliver pest control without chemical sprays.
1
Built a fluorescent biosensor using split-protein fusion to viral RNA-binding domains that reports relative dsRNA levels inside living bacteria
2
Tested multiple sensor designs in E. coli to find the version that best signals dsRNA accumulation
3
Used the optimized sensor to confirm boosted dsRNA output in engineered Serratia symbiotica, including a new RNase III deletion mutant