analytical-methods

1 article

Development of an Ultrahigh-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry Method for Multiclass Phytohormone Quantification Via Multifunctional Chemical Derivatization.

PubMed · 2026-05-27

Scientists developed a fast, sensitive test that can measure 27 different plant hormones simultaneously from just a tiny pinch of plant material in 10 minutes — far more efficient than previous methods.

27 phytohormones across multiple classes (including 18 gibberellins, abscisic acid, salicylic acid, auxin, and jasmonates) can be quantified in a single 10-minute run.

The method requires only 20 mg of plant material — roughly the weight of a few seeds — making it practical for small or precious samples.

Detection limits ranged from 0.04 to 29.9 nM, and the method was validated on three crop/food plants: tomato, maize, and cress.