Base editing in rice using nuclease-deactivated CRISPR/Cas-SF01.
Jia M, Xie Y, Wei C, Wang H, Xue L
Crispr
PubMedRice feeds more than half the world's population, and this more precise editing tool could accelerate breeding of varieties that survive drought, disease, or climate extremes — without the years of traditional crossbreeding.
Researchers developed a way to make very specific, small changes to the genetic code of rice plants — like fixing a single typo in a very long document. They used a modified version of CRISPR, a popular gene-editing technology, that doesn't cut the DNA but instead chemically converts one genetic 'letter' to another. The new approach worked about a third of the time and can now target more spots in the rice genome than before.
Key Findings
Average base editing efficiency of 33.3% achieved across multiple gene targets in rice
Both adenine (A-to-G) and cytosine (C-to-T) base edits were successfully demonstrated using the deactivated Cas-SF01 system
The PAM-relaxed SF01-IKRR variant expanded targeting range by enabling editing at 5'-NTN PAM sequences, not just standard PAM sites
chevron_right Technical Summary
Scientists used a gene-editing tool called CRISPR to make precise, targeted changes to the DNA of rice plants — achieving a one-in-three success rate and expanding the range of DNA locations that can be edited.
Abstract Preview
Adenine and cytosine base editing using dCas-SF01 and the 35S-CmYLCV-U6 composite promoter successfully introduced targeted base substitutions at multiple loci in rice, with average editing efficie...
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