New gene-editing toolkit reveals inflammation switch in immune cells
Jung H, Devant P, Ching C, Ota M, Dann E
Crispr
This study is about human immune cell engineering for cancer therapy and has no connection to plants, gardens, or botany.
Researchers made tiny particles that can carry gene-editing tools into human immune cells much more efficiently than before. Using this method, they found a gene called TNFAIP3 that acts like a brake on inflammation, and removing it made immune cells more aggressive against tumors. This could help scientists design better cell-based treatments for cancer in the future.
Key Findings
Developed a virus-like particle (VLP) system that delivers CRISPR tools into human monocytes, macrophages, and dendritic cells with high efficiency while preserving cell viability.
Created 'SLICeVLP,' combining VPX-lentivirus sgRNA delivery with VLP-based Cas9 delivery, to run large pooled genetic screens in human macrophages.
Identified TNFAIP3 as a key regulator of inflammation; deleting it created a persistently pro-inflammatory state that boosted cytotoxicity in CAR-macrophages used for cancer therapy.
chevron_right Technical Summary
Scientists built a new virus-like particle toolkit to efficiently edit genes in human immune cells, enabling large-scale screening that identified a gene (TNFAIP3) controlling inflammation, which could improve future cell-based cancer therapies.
Abstract Preview
Original paper
Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells.
Primary human myeloid cells hold promise for immunotherapies, yet efficient, scalable technologies for engineering and screening in these cells remain limited. Here we present a virus-like particle...
open_in_new Read full abstractAbstract copyright held by the original publisher.
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