Protocol for isolating plant-derived extracellular vesicles.
Chen Y, Xu L, Yu Y, Xu Z, Xiao S
Summary
6.8/10Scientists developed a detailed protocol for extracting and purifying plant-derived vesicles—tiny cellular packages involved in plant communication. This standardized method enables researchers worldwide to reliably study how plants send signals to each other.
Key Findings
Multi-stage isolation protocol combines differential centrifugation, ultrafiltration, ultracentrifugation, and sucrose density gradient centrifugation for efficient PEV enrichment
Method effectively removes cellular debris while maintaining PEV integrity and purity across diverse plant species
Protocol enables morphological characterization including particle size distribution and zeta potential measurements
Original Abstract
Plant-derived extracellular vesicles (PEVs) are nanosized, lipid bilayer-enclosed vesicles secreted by plant cells into the extracellular space, playing critical roles in intercellular communication. Here, we present a comprehensive and reproducible protocol for isolating PEVs from diverse plant species. Our integrated approach combines differential centrifugation, ultrafiltration, ultracentrifugation, and sucrose density gradient centrifugation to achieve efficient enrichment and purification of PEVs while effectively removing cellular debris. Finally, we characterize the isolated PEVs in terms of morphology, particle size distribution, and zeta potential.