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Removing a molecular brake made rice plants yield more

Jiang L, Zhao L, Wei J, Jiang B, Xiao Y

Crop Improvement

The rice on dinner tables worldwide could get a genetic tune-up from this discovery, since engineering the same protein tweak in rice plants meaningfully boosted their grain yield in tests.

Cells have a protein called ALKBH5 that normally works on RNA, but it has a flexible tail that keeps it from wandering onto DNA and stirring up trouble. When scientists snipped off that tail in mice, plants, and rice, the protein started loosening up tightly packed DNA and switching genes on. In rice, this trick made the plants produce more grain, and in a mustard-family lab plant it made roots grow better, showing this tiny protein tweak could become a real tool for breeding hardier, higher-yielding crops.

Key Findings

1

The C-terminal intrinsically disordered region (IDR) of ALKBH5 normally restricts its activity to mRNA and limits its access to chromatin in mouse embryonic stem cells.

2

Deleting the IDR redirects ALKBH5 to chromatin-associated noncoding RNAs, causing widespread chromatin opening, transcriptional activation, and reactivation of transposable elements; the same restraint mechanism applies to histone demethylases.

3

Applying IDR deletion to ALKBH5 and its plant homologs enhanced root growth in Arabidopsis and markedly increased grain yield in rice.

chevron_right Technical Summary

Scientists found a molecular 'brake' that keeps a gene-editing enzyme from making unwanted changes to DNA, and removing that brake in plants boosted root growth in a lab plant and significantly increased rice yield.

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Abstract Preview

Original paper

Intrinsically disordered regions restrain genomic targeting of RNA and histone demethylases in mammals and plants.

Intrinsically disordered regions (IDRs) are widely recognized as facilitators of chromatin regulation. However, their potential role as regulatory restraints remains poorly understood. Here we reve...

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Abstract copyright held by the original publisher.

hub This connects to 10 other discoveries — Arabidopsis, Rice crop-improvement, crispr, plant-signaling 5 related articles

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