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Breaking one rice gene made plants far more drought hardy

Seo HU, Jang CS

Crispr

If you've ever watched a garden crop wilt during a dry spell, this points to the exact molecular switch that could someday be bred into food crops to keep them alive on less water.

Researchers found a natural rice mutant that survives drought much better than normal rice, and traced the cause to a broken gene called OsRRK1. Normally this gene's protein blocks another protein from doing its job of helping the plant build up sugars; when that block is removed, the rice accumulates more sugar and handles dry conditions far better. The team confirmed this by editing the gene out entirely, which made plants more drought tolerant, while adding extra copies of the gene made plants wilt faster.

Key Findings

1

A frameshift mutation in OsRRK1 (from a single guanine deletion at glycine position 100) produced a drought-tolerant rice line called ditl5

2

OsRRK1 normally phosphorylates OsFBA6, an enzyme in the glycolysis pathway; disrupting this phosphorylation boosts glycolytic gene expression and soluble sugar accumulation

3

CRISPR/Cas9 knockout of OsRRK1 enhanced drought tolerance, while overexpression lines showed greater drought susceptibility, confirming the gene acts as a negative regulator

chevron_right Technical Summary

Scientists disabled a single gene in rice and made the plants noticeably tougher during drought, because that gene normally holds back a protein that helps rice cells store more sugar and cope with water stress.

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Abstract Preview

Original paper

Mutation in rice protein kinase OsRRK1 confers drought tolerance via non-phosphorylating fructose-1,6-bisphosphate aldolase 6.

Drought can severely restrict the productivity of rice, and thus gaining an understanding of its molecular basis is essential for developing viable stress-tolerant cultivars. In this study, to iden...

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Abstract copyright held by the original publisher.

hub This connects to 10 other discoveries — Rice crispr, climate-adaptation, crop-improvement +1 more 5 related articles

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