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A single cell-wall protein controls the first steps of plant embryo development

Luo A, Qiao Y, Li S, Wang X, Shi C

Plant Signaling

Seed germination rates and embryo viability in crops and garden plants depend on molecular signals most breeders can't yet see or manipulate, and this discovery opens a window into why some seeds abort even under ideal conditions.

Every plant starts life as a single fertilized cell that must divide in precise, organized ways to become a seedling. Researchers found a protein that sits in the cell wall of tobacco embryos right after fertilization and acts like a traffic controller, making sure those early divisions happen correctly. When they disabled this protein using gene-editing tools, the embryo's cells grew lopsided and stopped developing, and the seeds died.

Key Findings

1

The extracellular protein NtProRP1 localizes to the cell wall immediately after fertilization and is required for normal embryo cell division patterns in tobacco.

2

CRISPR-Cas9 knockout of NtProRP1 caused arrested embryos with irregular cell shapes and aborted seeds, demonstrating an essential developmental role.

3

Transcriptome profiling of isolated two-celled proembryos showed coordinated upregulation of genes for cellulose, hemicellulose, and pectin synthesis in mutants, implicating cell wall integrity signaling pathways including receptor-like kinases, MAPKs, Ca2+ flux, and phytohormones.

chevron_right Technical Summary

Scientists discovered a cell-wall protein in tobacco plants that acts as a molecular gatekeeper during the first cell divisions of a new embryo. Without it, embryos develop irregular shapes and die, revealing that the space outside plant cells plays a far more active role in early development than previously understood.

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Abstract Preview

Original paper

The extracellular regulatory network and key factors governing early embryogenesis in Nicotiana tabacum.

The plant extracellular space, including the cell wall, is a dynamic signaling compartment outside the plasma membrane that plays crucial roles in cell-cell communication and developmental coordina...

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Abstract copyright held by the original publisher.

hub This connects to 11 other discoveries — Tobacco plant-signaling, crispr, crop-improvement +2 more 5 related articles

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