Advancing the frontier of plant-based therapeutics: critical innovations in molecular farming and bioprocess Integration.
Gowtham K, Shanmugaraj B, Thangavel LS, Srinivasan A, Malla A
Summary
PubMedScientists are engineering plants to act as biological factories for producing medicines more efficiently by combining CRISPR gene editing with advanced biotechnology techniques. This approach could dramatically reduce the cost and complexity of manufacturing pharmaceuticals.
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Key Findings
CRISPR/Cas9-mediated pathway editing enables precise genetic modifications to enhance biopharmaceutical production in plant systems
Synthetic biology frameworks optimize protein yield and quality in plant-based expression systems
Integrated bioprocessing solutions improve downstream purification efficiency and reduce processing costs for plant-derived therapeutics
Original Abstract
Plant molecular farming (PMF) has emerged as a promising strategy for producing biopharmaceuticals and high-value biomolecules in plant systems. In this review, we present a comprehensive synthesis of current methodologies while introducing novel approaches to genetic transformation, protein expression, glycan engineering, and downstream processing. We offer in-depth analyses of recent advancements such as CRISPR/Cas9-mediated pathway editing, synthetic biology frameworks for optimizing protein yield and quality, and integrated bioprocessing solutions that enhance purification efficiency. Further, detailed case studies are discussed to illustrate actionable strategies, and future research directions are proposed to bridge current gaps. By focusing on transformative techniques and critical problem-solving perspectives, this review aims to guide researchers toward more effective and scalable PMF applications.
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