Prime editing: evolution of CRISPR-Cas system for a robust next-generation genome editing in plants.
Bargoti T, Nain V, Singh D
Crispr
The wheat, rice, and corn being developed with this technology could one day yield reliable harvests through droughts or disease outbreaks that would otherwise empty the shelves at your local co-op or farmers market.
Prime editing is like a very precise 'find and replace' tool for a plant's genetic code. Instead of making risky cuts to DNA the way older gene-editing tools did, it can swap out individual genetic letters, add small pieces, or remove them — all with much more control. Researchers have spent years improving this tool so it works well in crop plants like rice and corn, and this review maps out all those advances and what they mean for growing better food.
Key Findings
Prime editing can make all 12 possible single-letter DNA changes plus insertions and deletions without causing double-strand DNA breaks, making it safer and more precise than earlier CRISPR methods.
The technology has evolved through at least seven major generations (PE1 through PE7, plus TwinPE), with each iteration improving editing efficiency, accuracy, and the range of changes possible in plant cells.
Plant-specific adaptations — including codon optimization, redesigned guide RNAs, and tailored promoters — have been key to making prime editing work reliably across diverse crop species.
chevron_right Technical Summary
Scientists have reviewed how 'prime editing' — a next-generation gene-editing tool — is being refined and applied in plants. Unlike older methods, it can make precise DNA changes in crops without cutting both strands of DNA or needing external DNA templates, opening new doors for breeding better, more resilient plants.
Abstract Preview
Prime editing is a propulsive and versatile genome engineering technology that enables precise installation of all possible 12 base-to-base conversions, targeted insertions, deletions, and combinat...
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