long-read-sequencing
Long-read sequencing is a DNA sequencing technology capable of reading tens of thousands of base pairs in a single read, far exceeding the capabilities of traditional short-read methods. This approach is particularly valuable for plant science because plant genomes are often large and complex, containing extensive repetitive sequences that short-read technologies struggle to assemble accurately. Long-read sequencing enables more complete genome assembly, detection of structural variations, and comprehensive analysis of transcripts across diverse plant species.
PubMed · 2026-02-14
PAQman is a tool that helps researchers evaluate whether genome sequences are accurate and complete, without needing an existing reference sequence to compare against. It combines seven quality checks into one streamlined framework.
Tool measures 7 reference-free quality features: Contiguity, Gene content, Completeness, Accuracy, Correctness, Coverage, and Telomerality
Integrates multiple commonly used assessment programs alongside custom scripts in a unified framework
Requires only query genome assembly and underlying long-read sequencing data as inputs