diagnostics
Plant diagnostics is the systematic identification and analysis of diseases, nutritional deficiencies, pest infestations, and physiological disorders affecting plant health. In plant science research, accurate diagnostics are essential for understanding the underlying causes of plant stress and decline, enabling researchers to develop effective management strategies. This approach is fundamental to advancing plant breeding programs, disease resistance research, and sustainable agricultural practices.
open_in_new WikipediaPubMed · 2026-02-17
Researchers developed a CRISPR/Cas13a-based diagnostic test that rapidly detects groundnut bud necrosis virus without PCR amplification, offering a simple, field-deployable method for early virus detection in crops. The test is sensitive enough to detect virus at levels as low as 0.01 ng and works with crude plant extracts in the field.
CRISPR/Cas13a assay successfully detects GBNV without requiring PCR amplification, enabling faster diagnosis
Achieves high sensitivity with detection limit as low as 0.01 ng of viral RNA from both synthetic and crude field samples
Field-deployable using simple alkaline PEG buffer extraction from infected leaves, eliminating need for specialized laboratory equipment
